William A. Coetzee, DSc

Dr. Coetzee’s overall objective is to study the role of ion translocating mechanisms (ion channels and exchangers) in cardiovascular function during health and disease. His group utilizes multi-disciplinary approaches that take advantage of several methodologies, including biochemistry, molecular biology, electrophysiology, subcellular imaging of ion concentrations with confocal microscopy and genetically altered animal model systems. Their prime focus is the study of potassium channels, in particular the K ATP channel, which serves as a link between the cellular energy metabolism and excitability. Much of their work is directed at the cardiovascular system, including the cardiac myocyte, coronary artery and coronary endothelium. Dr Coetzee’s primary appointment is in Pediatrics, with joint appointments in Physiology & Neurosciences and Pharmacology.

Selected Publications:

  1. Kantor, P.F., W.A. Coetzee, E. Carmeliet, S.C. Dennis, and L.H. Opie. Reduction of ischemic K + loss and arrhythmias in rat hearts - Effect of glibenclamide, a sulfonylurea. Circulation Research 66: 478-485, 1990.
  2. Nakamura, T.Y., W.A. Coetzee, E. Vega-Saenz de Miera , M. Artman, and B. Rudy. Modulation of Kv4 channels, key components of rat ventricular transient outward K + current by PKC. American Journal of Physiology 273: H1775-H1786, 1997.
  3. Coetzee, W.A., Y. Amarillo, J. Chiu, A. Chow, T. McCormack, H. Moreno, M. Nadal, A. Ozaita, D. Pountney, E. Vega-Saenz de Miera, and B. Rudy. Molecular Diversity of K + Channels. Annals of the New York Academy of Sciences. 868: 233-285, 1999.
  4. Pountney, D.J., I. Gulkarov, E. Vega-Saenz de Miera, M. Saganich, B. Rudy, M. Artman, and W.A. Coetzee. Identification and cloning of TWIK-originated similarity sequence (TOSS): a novel human 2-pore K + channel principal subunit. FEBS Letters 450: 191-197, 1999.
  5. Coetzee, W.A., T. Wells, M. Avkiran . Anti-arrhythmic effects of levcromakalim in the ischaemic rat heart: a dual mechanism of action? European Journal of Pharmacology 402 (3) 263-274, 2000.
  6. Pountney, D.J., Z-Q Sun, L.M. Porter, M.N. Nitabach, T.Y. Nakamura, E. Rosner, M. Kaneko, T. Manaris, T.C. Holmes and W.A. Coetzee . Is the Molecular Composition of K ATP Channels More Complex Than Originally Thought? Biochemical and Electrophysiological Evidence for Heteromultimeric Assembly of the K ATP Channel Subunits Kir6.1 and Kir6.2. Journal of Molecular and Cellular Cardiology, 33 (8): 1541-1546, 2001.
  7. Nakamura, T.Y., D.J. Pountney, A. Ozaita, S. Nandi, S. Ueda, B. Rudy and W. A. Coetzee . A Role for Frequenin, a Ca 2+-Binding Protein, as a Regulator of Kv4 Currents. Proceedings of the National Academy of Sciences) 98 (22): 12808-12813, 2001. (cited 49 times)
  8. Haruna, T., H. Yoshida, T.Y. Nakamura, L-H Xie, H. Otani, T. Ninomiya, M. Takano, W.A. Coetzee, and M. Horie . a 1 -Adrenoceptor-Mediated Breakdown of Phosphatidylinositol 4,5-Bisphosphate Inhibits Pinacidil-activated K ATP Currents in Rat Ventricular Myocytes. Circulation Research 91: 232 – 239, 2002.
  9. Nakamura TY, Sturm E, Pountney DJ, Orenzoff B, Artman M, Coetzee WA. Developmental Expression of NCS-1 (Frequenin), a Regulator of Kv4 K + Channels, in Mouse Heart. Pediatric Research Feb 20, 2003.
  10. Meyers, M.B., A. Fischer, Y-J Sun, C. M. B. Lopes, T. Rohacs, T. Y. Nakamura, Y-Y. Zhou, P. C. Lee, R. A. Altschuld, S. A. McCune, W. A. Coetzee, G. I. Fishman . Sorcin Regulates Excitation-Contraction Coupling in the Heart. Journal of Biological Chemistry Aug 1;278(31):28865-71, 2003
  11. Yoshida H., Feig, J.E., Morrissey, A., Ghiu, I.A., Artman, M., and Coetzee, W.A.. K ATP Channels of Primary Human Coronary Artery Endothelial Cells consist of a Heteromultimeric Complex of Kir6.1, Kir6.2, and SUR2B Subunits. Journal of Molecular and Cellular Cardiology 37(4): 857-869, 2004.
  12. Morrissey, A., Yoshida, H., Rosner, E., Lanning, J., Lopez, G., Nakamura, T.Y., Giblin, J.P., Tinker, A., Artman, M., and Coetzee, W.A. Immunolocalization of K ATP Channel Subunits in Mouse and Human Cardiac Myocytes and the Coronary Vasculature. BMC Physiol. Jan 12;5 (1):1, 2005
  13. Srivastava, S., Lin, L., Li, Z., Coetzee, W.A., Skolnik, E.Y. The PI3P Phosphatase Myotubularin Related Protein 6 (MTMR6) is a Negative Regulator of the Ca 2+-Activated K + Channel KCa3.1. Molecular and Cellular Biology May;25(9):3630-8, 2005.
  14. Morrissey, A., Parachuru, L., Leung, M., Lopez, G., Nakamura, T.Y, Tong, X-Y., Yoshida, H., Srivastava, S., Dhar Chowdhury, P., Artman, M. and Coetzee, W.A. Expression of K ATP Channel Subunits during Perinatal Development in the Mouse Heart. Pediatric Research 58(2):185-192, 2005.
  15. Dhar Chowdhury, P., Harrell, M., Han, S., Jankowska, D., Parachuru, L., Morrissey, A., Srivastava, S., Liu, W., Malester, B., Yoshida, H., Coetzee, W.A. The glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase, triose phosphate isomerase and pyruvate kinase are components of the K ATP channel macromolecular complex and regulate its function. Journal of Biological Chemistry. Published online 16 September 2005, 10.1074/jbc.M508744200.